In this series of iCLIPs we treat cell with 1,6-hexanediol (1,6-HD) and other liquid droplet detergents in order to dissociate non-membranous subcellular compartments (eg. speckles, paraspeckles and stress granules). We are testing if the detergents capability of dissolving LCR-interactions could be used to minimise co-purification of contaminant RBPs during the pull-down step. Typical treatments are 5min 6% 1,6-HD PBS in live cells.
A meta collection that holds common Genome and Annotation files