Patrick Toolan-Kerr

3' QuantSeq of mESC lines that are either wildtype (WT), catalytic mutant (CM) or knockout (KO) for METTL3. Cells were either grown in 2iLIF, to maintain them in a naive pluripotent state, or for 2 days in N2B27, which induces spontaneous differentiation to a primed state, in which MEK signalling is active. Additionally conditions in which the WT cells were treated with a PARP inhibitor (Velaparib) or the KO cells were treated with a PARG inhibitor (PD00007273) for 24 hours were also added.